Table 2.

Cytokine secretion upon intramuscular administration of AAVlacZ and various adenovirusesa

GroupLeft legRight legIFN-γ (pg/ml)IL-10 (pg/ml)
MockAd5β-GalAAVMockAd5β-GalAAV
1AAVlacZ 00000.50.53.186
2AAVlacZ AdlacZ 027950400.83107709
3AAVlacZ + AdBglII017001.70.74803415
4AAVlacZ + AdALP 969905.10.886025101
  • a Lymphocytes from C57BL/6 animals harvested 10 days after infection were restimulated with purified β-galactosidase (β-Gal), AAVlacZ (AAV), UV-inactivated adenovirus type 5 (Ad5), or medium (Mock) for 48 h, and the supernatants were assayed for the secretion of IL-10 and IFN-γ by cytokine ELISA. The data are presented as the amount of cytokine secreted following stimulation with antigen. This experiment was repeated three times with identical results; representative data are presented.