Treatment^{a} | No. of S/field^{b} | No. of nuclei/synctium^{c} | % Nuclei associated with S |
---|---|---|---|

None | |||

9 hpi | 28 | 8 | 12.0 |

24 hpi | 34.5 | 48 | 88.0 |

NH_{4}Cl | |||

1 mM | 40.5 | 17 | 37.5 |

20 mM | 0 | 0 | |

Bfm A1 | |||

1 nM | 0 | 0 | |

100 nM | 0 | 0 | |

Virus^{d}
| |||

VSV_{IND}
| 36 | 57 | 94 |

VSV_{NJ/CA}
| 60 | 30 | 83 |

a At 9 hpi, fusion was detected only in untreated cells. All other values were determined at 24 hpi.

b The average number of giant cells or syncytia (S) was determined by counting giant cells in random fields of 20× micrographs of DAPI-stained monolayers. Only cells with more than four nuclei per cell were considered syncytia.

c Determined by using the formula (mock

_{n}− IN_{n})/N_{S}, where mock_{n}is the mean number of single nuclei determined from random fields in noninfected monolayers (mock_{n}= 1,875), IN_{n}is the mean number of single nuclei per random 20× field in infected monolayers (data not shown), and N_{S}is the mean number of syncytia per random field.d In a separate experiment, HECA2 cells were mock infected or infected with VSV

_{IND}or VSV_{NJ/CA}. Giant cell formation was determined as described above.