Table 1.

Inhibition of coreceptor function by CC chemokinesa

CCR5 region MutantMean relative inhibition (%) of coreceptor function withb:
MIP-1αMIP-1βRANTES
NtD2A819785
D11A 10 41 7
N13A1219392
E18A10010062
K22A 19 12 −11
K26A10097100
R31A −5 2 −16
ECL1H88A989599
Q93A8897114
D95A107112121
Q102A1079893
ECL2R168A908896
K171A/E172A 21 97107
H175A11910095
H181A 29 53 39
Y184A10268 36
Q188A957551
K191A/N192A 14 15 18
ECL3E262A100102100
R274A/D276A 48 36 33
  • a U87MG-CD4 cells were transiently lipofected with wt or mutant CCR5 and then infected with NLluc–JR-FL in the presence or absence of 2 μg of MIP-1α, MIP-1β, or RANTES/ml. Luc activity was measured 72 h later.

  • b Values are relative to inhibition by wt CCR5 (set at 100%) and are means of three independent experiments, each performed in quadruplicate. Values <50% of that observed with wt are in boldface.