Table 1.

Quantitation by slot blot hybridization of the relative amounts of EBV DNA in induced cells harboring wild-type or recombinant episomes and in virus pelleted from spent culture media

Sample typeSample dilutionAvg vol quantitationaWild type/recombinant ratio
Wild typeRecombinant
Cells1/10777,538619,1451.25
1/20462,640352,7951.31
1/40275,348208,4411.32
1/80164,243155,4241.06
Virus1/52,868,5652,604,9521.10
1/101,831,0331,762,5281.04
1/201,240,5191,133,0791.10
1/40645,572711,0450.91
  • a The intensity of hybridization was quantified by scanning with a Molecular Dynamics Storm 840 PhosphorImager and represents the average of duplicate slot blots.