Table 1.

Study subjects and samples evaluated by proviral DNA sequencinga

Subject designationDisease statusNo. of CD4+ T cellsTreatmentSourceNo. of sequences with V3 genotype
JOAIDS-KS467AZTPBMC10 NSI
NSMC5 NSI, 10 SI
PEAIDS45AZTPBMC8 NSI, 8 SI
NSMC22 NSI
613Asympt.419NonePBMC3 NSI, 5 SI
NSMC5 NSI, 10 SI
064Asympt.342AZT-ddIPBMC12 NSI
NSMC9 NSI
MAAsympt.402NonePBMC15 NSI
NSMC8 NSI
  • a The clinical condition, CD4+ T-cell number per cubic millimeter, and the antiretroviral treatment each subject was receiving on the day of sampling are reported. Mononuclear cells were prepared from the two specimens obtained, blood and semen, and the DNA was used as template for PCR, cloning in some cases, and DNA sequencing. The SI or NSI phenotype of each sequence examined was predicted based on the amino acid sequence in the V3 loop (19), and the numbers of each type encountered are reported. KS, Kaposi’s sarcoma; Asympt., asymptomatic; ddI, dideoxyinosine.