Table 1.

Infectivities of purified dpSVPs

Particle typePrepn no.P/PFUaRelative P/PFUb
Virion11401.0
22501.0
14-dpSVP12001.2
22001.2
31101.2
12-dpSVP14.0 × 105 3.4 × 10−4
23.7 × 105 3.7 × 10−4
33.2 × 105 4.2 × 10−4
  • a Particle (P)/PFU values for each virus were obtained by dividing P/milliliter by PFU/milliliter and are presented as averages for each particle type (n = 3). Each replicate measurement was derived from a different preparation of particles. Particle concentrations were determined by measuringA 260 and multiplying by the appropriate conversion factor (54). Viral infectivities were measured by plaque assay (22).

  • b Defined as the ratio of P/PFU for a preparation of virions to P/PFU of a given particle type (virion or dpSVP) derived from that preparation of virions.