Table 1.

Detection of viral antigen by immunofluorescence microscopy or IEM in axons and epidermal cells and isolation of virus in supernatants from the outside chamber

Time (h) after HSV infection (in IC)Axons in OCaEpidermal cellsaHSV isolation from supernatants (TCID50/ml)a
IEMIFIEMIFOCIC
120+ (<102)
16+++
28+++++++ (102–103)
32+++++++++
48+++++++++ (102–103)+ (104–105)
  • a 0, not performed; +, present (++, greater extent and intensity); −, absent; IF = immunofluorescence; TCID50, 50% tissue culture infective dose.