Table 4.

PCR results from 100-neuron samples from subject 8

Subject (side/sample no.)aPCR results for:
Neuronal cellsbNonneuronal cellsb
ORF21/QORF21/NORF21/QORF21/N
L/100
L/22+0
L/300
L/45+0
L/50+0
L/62+0
L/700
L/800
L/900
L/100+0
R/100
R/200
R/300
R/400
R/52+0
R/600
R/700
R/800
R/900
R/1000
  • a Side: L, left TG; R, right TG. Sample number: successive 100-neuron samples.

  • b ORF21/Q, primary PCR (40 cycles), followed by nested PCR (10 cycles). The number of VZV gene 21 copies after quantitation against standards is indicated. ORF21/N, primary PCR (40 cycles), followed by nested PCR (40 cycles). The presence (+) or absence (−) of VZV gene 21 at the highest possible sensitivity is indicated.