Table 2.

Integration of IN mutant HIV-1 complemented with HIV-2 IN protein in trans

Hygromycinresistant virusResult of cotransfection witha:
ControlVpr-IN2Vpr-IN
hy-SG3w t 24.3 × 104 18.9 × 104 20.6 × 104
hy-SG3S-IN 170.65 × 104 (22)3.0 × 104 (100)
hy-SG3D116A 531.0 × 104 (28)3.6 × 104 (100)
hy-SG3AA35A 232.1 × 104 (55)3.8 × 104 (100)
  • a Four micrograms of each of the hy-SG3wt, hy-SG3S-IN, hy-SG3D116A, and hy-SG3AA35A clones (40) were separately cotransfected into 293T cells with 2 μg of the VSV-G envplasmid and 2 μg of either the pLR2P (control), Vpr-IN2, or Vpr-IN expression plasmids. Numbers indicate the mean number of hygromycin-resistant colonies per 100-ng equivalent (p24 antigen) of input virus. The numbers in parentheses indicate the number of hygromycin-resistant colonies relative to the number produced by Vpr-IN-complemented SG3S-IN virions, which was arbitrarily set to 100. These results are representative of three independent experiments.