Table 1.

Surface expression of HN cytoplasmic tail-truncated HN proteinsa

Protein expressedSurface expression efficiency
% of positive cellsRelative mean fluorescence intensity
HN93.01.00
HNΔ2-394.10.75
HNΔ2-594.70.90
HNΔ2-792.60.94
HNΔ2-994.30.91
HNΔ2-1194.00.84
HNΔ2-1394.10.78
HNΔ2-1593.80.73
HNΔ2-1725.50.03
None (mock transfected cells)0.01
  • a HN proteins were synthesized in CV-1 cells with the vaccinia virus T7 expression system. Surface expression of HN was determined by flow cytometry at 16 h posttransfection with a cocktail of two HN-specific MAbs followed by a fluorescein isothiocyanate-conjugated secondary antibody. Values shown are averages of results from three experiments.