Table 1.

Virus titers after infection with vectors containing 114- to 701-bp direct repeats separated by spacer fragments of various lengths

VirusDistance between homologous sequences (bp)aTiter (CFU/ml)b
G418HAT
KD-HTnotTK7017.3 × 104 NDc
KD-HT98TK7993.7 × 103 3.9 × 103
JT-HT213TK9145.0 × 103 5.4 × 103
KD-HT304TK1,0055.2 × 103 5.5 × 103
JT-HT387TK1,0883.1 × 103 7.0 × 103
KD-HT496TK1,1976.6 × 103 8.7 × 103
KD-HT599TK1,3003.3 × 103 4.6 × 103
KD-HT706TK1,4071.1 × 103 2.0 × 103
JT-HT803TK1,5046.3 × 103 9.6 × 103
KD-HTpTKd 1,5196.0 × 103 1.1 × 104
KD-HT844TK1,5459.3 × 104 1.0 × 105
KD-HT1299TK2,0005.5 × 103 ND
KD-HT1601TK2,3025.4 × 103 ND
KD-HT1844TK2,5453.6 × 103 4.6 × 103
KD-HT2310TK3,0117.2 × 103 ND
KD-HT3322TK4,0234.4 × 103 3.8 × 103
KD-HT3474TK4,1752.1 × 105 2.4 × 105
KD-HTT4*K701ND2.7 × 105
KD-HTpT4*K1,519ND1.8 × 104
KD-HT1TK1141.6 × 105 1.1 × 104
KD-HT2TK2256.7 × 104 1.8 × 104
KD-HT4TK3499.5 × 104 1.6 × 104
KD-HTp1TK9324.4 × 104 2.6 × 103
KD-HTp2TK1,0433.2 × 104 1.2 × 103
KD-HTp4TK1,1671.9 × 105 4.7 × 103
KD-HTTK34747014.1 × 101 1.2 × 102
KD-neoHTTK7014.3 × 103 ND
  • a Length of one direct repeat plus length of the spacer fragment between the direct repeats.

  • b Viral titers shown are the Average of two to six independent experiments.

  • c ND, not determined.

  • d Data from reference 6.