Table 4.

BAL cell types at days 3 and 6 after challenge with B1 or CP52 in mice vaccinated with formalin-inactivated virus

TreatmentaCell typebH&E staining
Day 3 p.i.Day 6 p.i.
ImmunizationChallengeRange, % positiveMedian % (Pc)Range, % positiveMedian % (P)
FI-B1B1MAC30–4235 (<0.01)6–1514 (<0.01)
PMN2–86 (>0.01)3–86 (>0.10)
EOS30–4032 (<0.01)50–6250 (<0.01)
LYM25–3027 (>0.05)25–3830 (<0.05)
FI-B1CP52MAC68–7572 68–7575 
PMN2–95 5–116 
EOS2–105 1–76 
LYM15–2218 10–1813 
FI-CP52B1MAC80–8883 (>0.10)85–9289 (>0.10)
PMN0–52 (>0.10)1–64 (>0.10)
EOS2–86 (>0.10)0–42 (>0.10)
LYM5–119 (>0.10)3–105 (>0.10)
FI-CP52CP52MAC78–8581 87–9694 
PMN3–95 1–62 
EOS0–62 00 
LYM10–1812 2–84 
FI-PIV3B1MAC82–9087 (>0.10)58–7270 (>0.10)
PMN1–63 (>0.10)11–1811 (>0.10)
EOS0–42 (>0.10)8–148 (>0.10)
LYM6–128 (>0.10)6–1211 (>0.10)
FI-PIV3CP52MAC76–8880 52–7672 
PMN2–66 6–1110 
EOS2–105 6–1913 
LYM6–149 6–185 
  • a Mice were i.n. infected with 104 PFU of virus or i.p. administered 104 PFU equivalents of formalin-inactivated virus.

  • b BAL was collected and analyzed by H&E staining for percent MAC, PMN, EOS, and LYM.

  • c The nonparametric Mann-Whitney analysis was used to test for significant differences in percent cells between B1- and CP52-infected mice within each grouping. Total cell numbers in BAL of B1- and CP52-infected mice ranged from 3 × 105 to 6 × 105/ml and from 3 × 105 to 6 × 105/ml, respectively.