Table 1.

Frequencies of CD8+ cells specific for the dominant and subdominant Sendai virus epitopes as measured by MHC tetramer staininga

Cell source (no. of days post-infection) and virusTotal CD8+cellsCD8+ CD44hicellsCD8+ CD62Llo cells
SV-NP/KbSV-NP/DbFlu-NP/DbSV-NP/KbSV-NP/DbFlu-NP/DbSV-NP/KbSV-NP/DbFlu-NP/Db
BAL fluid (10)
 Sendai70.71.10.270.80.90.268.90.80.0
 Influenza1.00.215.31.00.117.11.10.120.6
Spleen (19)
 Sendai5.90.30.213.90.50.124.40.50.0
 Influenza0.50.01.40.70.42.60.80.32.4
Spleen (36)
 Sendai4.02.20.012.01.90.228.52.10.2
 Influenza0.30.51.00.60.62.32.00.67.3
Spleen (89)
 Sendai2.11.20.06.70.70.23.00.70.0
  • a C57BL/6 mice were infected with either 500 50% egg-infectious doses of Sendai virus or 240 hemagglutination units of A/HK-x31. BAL fluid was analyzed 10 days postinfection, at the peak of the acute CD8+ T-cell response. Spleen cells were analyzed at 19, 36, and 89 days postinfection to measure memory CTL frequencies. Staining was performed with MHC tetramers consisting of Db molecules folded with either the Sendai virus NP324–332 peptide (SV-NP/Db) or the influenza virus NP366–374 peptide (Flu-NP/Db) or Kb molecules folded with the Sendai virus NP324–332 peptide (SV-NP/Kb). Data are presented as percentages of tetramer-positive cells among total CD8+, CD8+ CD44hi, or CD8+CD62Llo T cells.