Table 3.

Analysis of ex vivo CD8+ T-cell responses specific for autologous EBV-LCL eventually infected with vaccinia virus recombinants for various HIV-1LAI genes

PatientMediumEBVVaccinia virus
FrequencyaCD28bFrequencyCD28Wild typeEnvGagPolNef
FrequencyCD28FrequencyCD28FrequencyCD28FrequencyCD28FrequencyCD28
C03M202651,1617388371 6,244 24 4,728 31 4,450 23 3,914 28
P04258222,142201,66324 7,674 12 3,296 16 2,555 181,28221
Z05087691,57271868591,14141 1,970 29 2,270 26 2,866 37
Z077224153,494601,74952 7,726 15 7,492 21 18,000 10 5,060 27
Z100188265,976502,81042 6,018 18 20,225 16 9,825 22 2,551 38
Z108333621,39274760621,14739 7,867 17 2,960 27 923 48
  • a Number of CD8+ T cells secreting IFN-γ in response to antigen per 106 total CD8+ lymphocytes.

  • b Percent CD28 expression on IFN-γ+ CD8+ T lymphocytes. Generally both a higher frequency of IFN-γ-secreting cells and a lower expression of CD28 attest to the specificity of the responses directed against EBV-LCL infected with vaccinia virus recombinants for HIV genes compared with those against EBV-LCL infected with wild-type vaccinia virus (values in boldface).