Table 6.

BAL cell types at day 3 and 6 after challenge of control mice with B1 or CP52

TreatmentaH&E stainingFlow cytometry
Cell typebDay 3 p.i.Day 6 p.i.Cell phenotypedDay 3 p.i.Day 6 p.i.
ImmunizationChallengeRange, % positiveMedian % (Pc)Range, % positiveMedian % (P)Range, % positiveMedian % (P)Range, % positiveMedian % (P)
pcDNAB1MAC85–9288 (>0.10)78–9694 (>0.10)CD325–360 (>0.10)33–4235 (<0.01)
PMN0–52 (>0.10)1–53 (>0.10)B2201–105 (>0.10)1–168 (>0.10)
EOS0–22 (>0.10)0–21 (>0.10)DX50–85 (<0.01)3–127 (<0.01)
LYM5–118 (>0.10)0–22 (>0.10)RB6-8C52–105 (<0.05)1–96 (<0.01)
CD11b8–1510 (<0.01)2–106 (>0.10)
pcDNACP52MAC80–9587 70–9492 CD330–4034 12–1714 
PMN1–53 0–123 B2200–42 4–106 
EOS001–61 DX530–3835 37–5448 
LYM2–1210 1–54 RB6-8C58–2822 19–2522 
CD11b25–4038 3–106 
  • a Mice were i.n. infected with 104 PFU of virus or with pcDNA as described in Materials and Methods.

  • b BAL was collected and analyzed by H&E staining for percent positive MAC, PMN, EOS, and LYM.

  • c The nonparametric Mann-Whitney analysis was used to test for significant differences in percent cells between B1- and CP52-infected mice within each grouping. Total cell numbers in BAL of B1- and CP52-infected mice ranged from 2.0 × 105to 3.5 × 105/ml and from 2.2 × 105to 3.5 × 105/ml, respectively.

  • d BAL was collected and analyzed by flow cytometry for percent CD3+ T cells, B220+ B cells, DX5+ NK cells, RB6-8C5 neutrophils, and CD11b+ PMN.