Table 2.

Fraction of cells producing mutant virus

Relevant genotypeVirusVirus-producing cells (%)a
Asyncb (n)S phase (n)
Wild type dl30995 (8)95 (4)
E1B 55-kDa-mutant dl152021 (8)58 (4)
orf6-mutant dl35535 (3)60 (2)
orf3-mutant inorf395 (3)96 (1)
orf3-orf6-mutant inorf3/inorf627 (3)22 (2)
dl355*/inorf310 (3)14 (2)
E4-deletion dl366* 3 (2)ND
dl366*+orf1-224 (1)24 (1)
dl366*+orf328 (2)40 (1)
dl366*+orf419 (1)14 (1)
  • a HeLa cells that were clearly infected were scored for the presence or absence of virus particles. At least 200 cells per experiment were counted, and each value represents an average of cells producing virus. All measurements from multiple experiments (n > 1) were within ±8%. n, number of independent experiments. ND, not determined.

  • b G1 cells were used fordl520, inorf3, anddl355*/inorf3 infections instead of asynchronous (Async) cells.