Table 1.

BAL cell types by H&E staining and flow cytometry at days 3 and 6 after B1 or -CP52 primary infection

InfectionaH&E stainingFlow cytometry
Cell typebDay 3 p.i.Day 6 p.i.Cell phenotypedDay 3 p.i.Day 6 p.i.
Range, % positiveMedian % (Pc)Range, % positiveMedian % (P)Range, % positiveMedian % (P)Range, % positiveMedian % (P)
B1MAC80–8884 (>0.10)78–9086 (>0.10)CD338–4442 (>0.05)30–4436 (>0.05)
PMN2–64 (>0.10)0–41 (>0.10)B2204–106 (>0.10)9–1612 (<0.05)
EOS1–52 (>0.10)0–53 (>0.10)DX52–85 (<0.01)8–128 (<0.01)
LYM4–1510 (>0.10)2–1210 (>0.05)RB6-8C50–42 (<0.01)8–128 (<0.01)
CD11b5–128 (<0.01)6–107 (<0.01)
CP52MAC75–9085 75–8887 CD327–3230 19–2523 
PMN2–84 2–114 B2201–42 2–54 
EOS0–31 1–84 DX518–2520 24–3528 
LYM6–1610 2–55 RB6-8C514–1815 18–2522 
CD11b20–242215–24 21 
  • a Mice were i.n. infected with 104 PFU of virus.

  • b BAL was collected and analyzed by H&E staining for percent MAC, PMN, EOS, and LYM.

  • c The nonparametric Mann-Whitney analysis was used to test for significant differences in percent cells between B1- and CP52-infected mice within each grouping. Total cell numbers in BAL of B1- and CP52-infected mice ranged from 4 × 105 to 4.5 × 105/ml and from 3.8 × 105 to 5.5 × 105/ml, respectively.

  • d BAL was collected and analyzed by flow cytometry for percent CD3+ T cells, B220+ B cells, DX5+ NK cells, RB6-8C5 neutrophils, and CD11b+ PMN.