Table 2.

Protein/nucleic acid ratios used in‚ÄČassembly

SampleMolar ratioaAssembly
48-base oligonucleotide20:1No
10:1No
2:1Yesb
1:1Yes
0.1:1Yes (few)
0.01:1No
Genomic RNA>1,000:1No
1,000:1Yes
500:1Yes
400:1Yesb
200:1Yes (few)
100:1No
  • a Protein:DNA oligonucleotide for the 48-base oligonucleotide and protein: RNA for genomic RNA.

  • b Under the optimum assembly conditions for the indicated nucleic acid.