Table 1.

Epitopic specificities found in five immunized macaques

Effector cellsa from macaqueTarget cellsb% Specific lysisc at the E/T ratiod of:
100:133:111:14:1
92109None2521
Nef 169-17889e 75
None14115
Nef 215-225413622
None19973
Gag 266-2754024166
None1411115
Nef 101-110262216
Nef 128-1362823
None41344127
Nef 116-12657454834
92129None8320
Nef 128-1364634289
Nef 201-2112216165
Nef 211-2191916104
None524430
Nef 169-178655433
92125None38342519
Nef 169-17886877054
92127None2821
Nef 116-1264332
92105No peptide2214122
Nef 128-1363424135
  • a CTL cell lines were obtained from PBMCs of the five immunized macaques following specific stimulation with the seven long peptides in vitro.

  • b Target cells were autologous B-LCLs immortalized by the herpesvirus papio and incubated with short peptides (10 μM).

  • c Target cells (5 × 103) were labeled with 51Cr and incubated for 4 h with various numbers of target cells.

  • d E/T ratio, effector-to-target ratio.

  • e CRT was considered positive if the specific51Cr release observed in the presence of peptide-pulsed target cells exceeded by 10% that observed on B-LCLs without peptide at two E/T ratios.