Table 1.

Purification summary for VP5, triplexes, and pUL80.5-H

Purification step% of total protein presenta
VP5TriplexpUL80.5-H
Clarified lysate171124
Ammonium sulfate precipitation414846
Anion-exchange chromatography79NANA
Cation-exchange chromatographyNA95NA
Sucrose density gradient96NA93
  • a The percentage of each capsid protein present was determined by densitometric scanning of a Coomassie-blue-stained SDS-polyacrylamide gel. NA, not applicable.