Table 3.

RNase sensitivity of the genomic and antigenomic GBV-C RNA strands in pellets from culture supernatants of PBMC experimentally infected with GBV-C

Treatment used on pellet derived from culture supernatantaGBV-C RNA strand-specific RT-PCR resultb
NP-40RNase ARNase inhibitorGenomic strandAntigenomic strand
++
+++
+++
+++
+++++
  • a +, present in reaction mixture; −, absent from reaction mixture.

  • b +, product obtained; −, no product obtained.