Table 1.

Stimulation of transgene expression by the WPRE in arrested cells

VectorLuciferase expression in 293T cellsa
DividingGamma irradiatedb
HR′CMV-Luc1,291,608 ± 332,1111,037,861 ± 366,257
HR′CMV-Luc-Was270,129 ± 13,035235,980 ± 30,876
HR′CMV-Luc-W10,977,543 ± 2,547,09110,455,054 ± 3,065,829
  • a Reported as relative light units per nanogram of p24 (mean ± standard error of the mean). For each vector type, 10 μl of two independently produced stocks representing 1.5 to 4.5 ng of p24 was used to transduce in parallel and in duplicate 2 × 105 cells. The estimated MOI was 0.01 to 0.03.

  • b 8,000 rads delivered by a 3-min exposure to a60Co source.