Table 1.

Effect of human serum on the CD14-dependent inhibition of DV infection of MO/Mφ by LPSa

Expt no.Virus titer in PFU/ml (% inhibition) with (+) or without (−):
−ΔAHS+ΔAHS
−LPS+LPS+MY4, +LPS+MoS3, +LPS−LPS+LPS+MY4, +LPS+MoS39, +LPS
12.31 × 105 8.46 × 104 (63.4)2.20 × 105 (4.7)2.46 × 105 (−6.5)6.53 × 104 3.50 × 103 (94.6)5.07 × 104 (22.4)6.67 × 104 (−2.1)
22.74 × 105 9.23 × 104 (66.3)2.43 × 105 (11.2)ND1.14 × 105 1.03 × 104 (90.9)1.21 × 105 (−6.1)ND
  • a Seven-day-old primary human MO/Mφ obtained from two different donors (i.e., experiments 1 and 2) were used. The cells were infected with DV at an MOI of 3 PFU per cell. Viral adsorption was performed in serum-free α-MEM (−ΔAHS) or α-MEM containing 3% ΔAHS (+ΔAHS), either with or without 5 μg of LPS per ml (+LPS and −LPS, respectively) for 2.5 h. Before coincubation of LPS and DV, some cultures were pretreated overnight with 20 μg of MY4 per ml (+MY4) or 30 μg of MoS39 (+MoS39) per ml. After 44 h of infection, culture supernatants were harvested and assayed to determine the infectious-DV titers. ND, not determined.