Table 2.

Mutation rate of A/Swine/Germany/2/81

Clone no.Plaque inhibition assay result (PFU/ml)Allantoic fluid (ml)Population size (PFU)aMutant frequencybMutation rate (bp/site/replication)cQuotient (control/ amantadine)Quotient × 4Quotient × 4/ genome length
ControlAmantadine
19.2 × 108 1.2 × 105 13.01.2 × 1010 1.3 × 10−4 1.9 × 10−6 7.7 × 103 3.1 × 104 2.3
26.0 × 108 2.6 × 105 9.05.4 × 109 4.3 × 10−4 6.7 × 10−6 2.3 × 103 9.2 × 103 0.7
32.3 × 108 1.8 × 105 13.03.0 × 109 7.8 × 10−4 1.2 × 10−5 1.3 × 103 5.1 × 103 0.4
46.0 × 108 2.6 × 105 11.06.6 × 109 4.3 × 10−4 6.6 × 10−6 2.3 × 103 9.2 × 103 0.7
51.0 × 109 2.0 × 105 12.01.2 × 1010 2.0 × 10−4 3.0 × 10−6 5.0 × 103 2.0 × 104 1.5
62.2 × 108 8.0 × 104 10.72.4 × 109 3.6 × 10−4 5.8 × 10−6 2.8 × 103 1.1 × 104 0.8
74.2 × 108 1.4 × 105 9.33.9 × 109 3.3 × 10−4 5.2 × 10−6 3.0 × 103 1.2 × 104 0.9
84.0 × 107 4.0 × 104 8.63.4 × 108 1.0 × 10−3 1.8 × 10−5 1.0 × 103 4.0 × 103 0.3
96.0 × 107 1.8 × 104 9.65.8 × 108 3.0 × 10−4 5.2 × 10−6 3.3 × 103 1.3 × 104 1.0
103.2 × 107 2.0 × 104 10.53.4 × 108 6.3 × 10−4 1.1 × 10−5 1.6 × 103 6.4 × 103 0.5
111.0 × 108 1.2 × 104 6.06.0 × 108 1.2 × 104 2.1 × 10−6 8.3 × 103 3.3 × 104 2.5
124.0 × 108 4.0 × 104 13.55.4 × 109 1.0 × 10−4 1.5 × 10−6 1.0 × 104 4.0 × 104 2.9
132.8 × 108 2.4 × 105 10.83.0 × 109 8.6 × 10−4 1.4 × 10−5 1.2 × 103 4.7 × 103 0.3
144.0 × 107 8.0 × 103 8.83.5 × 108 2.0 × 10−4 3.5 × 10−6 5.0 × 103 2.0 × 104 1.5
151.2 × 108 4.8 × 104 9.01.1 × 109 4.0 × 10−4 6.7 × 10−6 2.5 × 103 1.0 × 104 0.7
161.3 × 108 1.6 × 104 11.91.5 × 109 1.2 × 10−4 2.0 × 10−6 8.1 × 103 3.3 × 104 2.4
171.4 × 108 9.6 × 104 9.81.4 × 109 6.9 × 10−4 1.1 × 10−5 1.5 × 103 5.8 × 103 0.4
Average3.1 × 108 1.0 × 105 3.5 × 109 4.2 × 10−4 6.8 × 10−6 3.9 × 103 1.6 × 104 1.2
  • a Product of titer from control and amount of allantoic fluid.

  • b Quotient of titers from plaque inhibition assays with amantadine and control.

  • c Rate for each clone calculated from mutant frequency and population size as described in Materials and Methods.