Table 2.

Intracellular localization of p53 in A549 cells infected with recombinant adenovirus

Time (h) postinfectionIntracellular localization of p53 witha:
Virus 300 wt (+/+)E4 in orf3 (−/+)dl366* (−/−)dl366*+orf3 (+/−)
8Detectable only in a small proportion of the cells, diffusely in the nucleusDetectable only in a small proportion of the cells, diffusely in the nucleusAlmost exclusively in the cytoplasmic clustersDiffusely in the nucleus
12NDNDAlmost exclusively in cytoplasmic clustersIn the nucleus, with some concentration in emerging replication centers
18NDNDAlmost exclusively in cytoplasmic clusters; in the replication centers of a few cellsIn the nucleus, mostly in replication centers
24NDNDAlmost exclusively in cytoplasmic clusters; in the replication centers of a few cellsIn the nucleus, mostly in replication centers
  • a The presence (+) or absence (−) of E4orf3/E4orf6 is indicated in parentheses. wt, wild type; ND, not detectable.