Table 2.

Primers used for PCR amplification of the VP1 region of enteroviruses

PrimerSequenceaGenePositionb
008GCRTGCAATGAYTTCTCWGTVP32411–2430
009NGCNCCDGATTGNTGSCC2A3409–3391
011GCICCIGAYTGITGICCRAA2A3408–3389
012ATGTAYGTICCICCIGGIGGVP12951–2970
013GGIGCRTTICCYTCIGTCCAVP13051–3032
019ACRTGICIIGTYTGCATIGTVP12676–2657
035AWITTYTAYGAYGGITGGVP13098–3115
036TAIAIIGTICCCATRTTRTTVP13201–3182
040ATGTAYRTICCIMCIGGIGCVP12951–2970
041GGIGGIGGRTCIGTIAKYTTVP13054–3035
045GAIGARAAYCTIATIGARACVP12648–2667
046CCCATIAKRTCIATRTCCCVP12820–2801
050GTRCTYACIAIIAGRTCYCT2A3513–3494
051TSAARYTGTGCAARGACACVP32429–2448
052STGYCCAGATTTCAGTGTVP32413–2430
053GGNACNCAYRTNATHTGGGAVP32216–2235
054GCCITRTTITGRTGICCRAA2A3408–3389
055GGIACICAYRTIRTITGGGAVP32216–2235
  • a Sequences are shown 5′ to 3′ with standard codes.

  • b Nucleotide sequence coordinates are given relative to the published sequence of PV1-Mahoney (34, 55) for orientation only. For most of the primers, specificity is limited to a subset of the human enteroviruses; not all virus-primer pair combinations have been tested.