Table 1.

Intracellular localization of the E1B-55-kDa protein in A549 cells infected with recombinant adenovirus

Time (h) postinfectionIntracellular localization of E1B-55-kDa witha:
Virus 309 (+/+)Virus 355 (+/−)E4 in orf3 (−/+)dl366*(−/−)dl366*+orf3 (+/−)
6Cytoplasmic clusters; low levels of nucleoplasmic staining in some cellsCytoplasmic clustersCytoplasmic clusters; low levels of nucleoplasmic staining in some cellsCytoplasmic clustersCytoplasmic clusters
9Cytoplasmic clusters; nuclear tracks; some diffusely nucleoplasmic stainingCytoplasmic clusters; nuclear tracksCytoplasmic clusters; some diffusely nucleoplasmic stainingCytoplasmic clustersCytoplasmic clusters; nuclear tracks
12Diffusely nucleoplasmic staining; some cytoplasmic clustersCytoplasmic clusters; nuclear tracksDiffusely nucleoplasmic staining; some cytoplasmic clustersCytoplasmic clustersCytoplasmic clusters; nuclear tracks
18In the nucleus, mostly around the replication centersIn the nucleus, mostly around the replication centersNot assayedCytoplasmic clustersIn the nucleus, mostly around the replication centers
24In the nucleus, almost exclusively around the replication centersIn the nucleus, almost exclusively around the replication centersNot assayedCytoplasmic clustersIn the nucleus, almost exclusively around the replication centers
  • a The presence (+) or absence (−) of E4orf3/E4orf6 is indicated in parentheses.