Table 1.

Cell surface expression of CD8-TM-CD hybrids in HeLa cells

VectorCell surface expression of CD8-TM-CD hybridsa (mean ± SEM)
% GFP+CD8+ cellsbCD8 fluorescence intensityc
pRSV-GFP1.5 ± 0.21.5 ± 0.2
pJ.CNstop94.3 ± 0.931.2 ± 3.6
pCD8-HIV61.7 ± 0.87.1 ± 0.2
pCD8-HIV-Y712A74.2 ± 1.011.2 ± 0.4
pCD8-SIV51.8 ± 2.37.0 ± 0.4
pCD8-SIV-Y721A61.2 ± 2.79.0 ± 0.5
pCD8-HTLV79.8 ± 3.212.5 ± 1.4
pCD8-HTLV-Y476S81.8 ± 0.912.9 ± 1.3
pCD8-HTLV-Y479S94.9 ± 0.320.8 ± 2.5
pCD8-HIVΔ57.3 ± 3.57.2 ± 0.5
pCD8-HIVΔ-Y712A92.0 ± 1.515.5 ± 1.3
pCD8-SIVΔ50.9 ± 6.98.2 ± 0.5
pCD8-SIVΔ-Y721A89.4 ± 1.319.2 ± 2.6
  • a Average of three independent experiments. After 48 h, surface expression of CD8 was analyzed in GFP+ cells by flow cytometry.

  • b Percentage of GFP+ cells expressing CD8 hybrids at the cell surface.

  • c CD8 fluorescence intensity of GFP+ cells expressing CD8 hybrid at the cell surface.