Table 2.

Immunophenotypic characterization of cell lines

Cell lineRelative fluorescence intensitya
CD3bCD4CD8αCD8βCD28TCR1TCR2TCR3MHC-IIc,dIgMcBuc
MDCC-UD01+++++++++++++
MDCC-UD02++++++++
MDCC-UD03+/−+++++/−++
MDCC-UD13+/−+++++/−++++
MDCC-UD14++++++++++++++
MDCC-UD22++++NDND+++
MDCC-UD24++++++++
MDCC-UD25++++NDND+++++
MDCC-UD27++++NDND+++
MDCC-UD28++++++++++++++
MDCC-UD29++++NDND+++
MDCC-MSB1e ++++++++/−
RECC-CU60f NDND++++++
RECC-CU91++++++
  • a Number of plus signs indicates relative fluorescence intensity determined by direct staining methods; −, no detectable expression by direct or indirect staining methods; +/−, surface expression detectable only via indirect staining methods; ND, not determined.

  • b Anti-chicken T-cell antibody obtained from SBT.

  • c Anti-chicken MHC-II (P2M11), IgM/IgD, and Bu-1a monoclonal antibodies obtained from H. Lillehoj.

  • d Anti-chicken MHC-II (CIa-1) antibody obtained from D. Ewert.

  • e Originally described in reference3; obtained from S. Cloud.

  • f Originally described in reference77; obtained from K. A. Schat.