Table 1.

Particle numbers and infectious titers of representative Ad preparations

VirusCsCl-purified prepnCell lineParticles/mlaPFU/mlbParticle/PFU ratioFiber source
Ad5.βgal.wt1211B7.4 × 1011 7.5 × 1010 10Ad chromosome
2211B3.0 × 1011 5.0 × 109 60Ad chromosome
Ad5.βgal.ΔF3211B7.7 × 1011 3.5 × 108 2,200Packaging cells
4211B1.9 × 1012 2.3 × 109 808Packaging cells
5293 4.5 × 1011 9.5 × 106 47,400None
6293 3.4 × 1011 3.5 × 107 9,700None
  • a Calculated from viral protein concentration (1 μg of protein = 4 × 109particles).

  • b Assayed by plaquing on 211B cells (see Materials and Methods).