Table 2.

Vmw110 mutant viruses used in this study

Virus strainVmw110 residues, deletions, or substitution(s)HAUSP bindingaND10 colocali-zationbND10 disruptioncMultimerd
17+1–775 (wild type)++++
FXEΔ106–149+++
E52X1–593
E58X1–632(−)
A8X1–646(−)
D12Δ594–632+++
A78Δ592–647++(+)
M1R623L/K624I++(+)
M2R623LReduced++(+)
M4K620I++(+)
  • a The ability to bind HAUSP in coimmunoprecipitation experiments from virus-infected cell extracts as shown in Fig. 4. The data for viruses FXE and D12 were taken from references 26 and 27.

  • b The ability of the mutant protein to colocalize with PML in ND10 as shown in Fig. 6. The data for viruses not shown in Fig. 6 were taken from references 11,24, and 27.

  • c Disruption of ND10 in HEp-2 cells 5 h after the start of infection. See Fig. 6.

  • d The ability of Vmw110 to multimerize and the effects of mutations of strains FXE, E52X, and D12 have been determined experimentally (30). Parentheses indicate that the mutations in these proteins affect sequences in the mapped multimerization domain (4, 30).