Table 1.

Characteristics of the GST fusion proteins including C-terminal portions of Vmw110 used in this study

PlasmidVmw110 amino acid residuesaHAUSP bindingb
pGEXE52594–775+
pGEXE4615–775+
pGEXE9618–775+
pGEXE58633–775
pGEXE52Pm1I594–713+
pGEXE52RsaI594–680+
pGEXE52AvaI594–646+
pGEXE23X594–638Weak
pGEXE58X594–633
  • a Vmw110 residues fused to the C-terminal end of GST as explained in Materials and Methods.

  • b The ability of the fusion proteins to bind to HAUSP in vitro as shown in Fig. 1. The result for pGEXE58 is taken from reference 29.