Table 1.

Primers used to generate ORF 1 clones of VR-2332

PrimerSequenceLocation
658P1/5′-CAGGAGCTGTGACCATTGGC45–64
/658P45′-GAAGGGTGTTTCTGTGCAGC60–79
5′5500/5′-CAAGACCCAATTCTGCACG5848–5863
5′6050/5′-GCGCCCCTCAGGCCAGTTTT6049–6068
/3′58005′-TGGCTGCCGACCTTCAC6124–6140
5′5800/5′-GGCAGCCACATAATTAAAGACATA6149–6172
/3′62005′-ACTGCACCGAGGCTTGAA6458–6475
LAF2/5′-GCTTTGTATCTGCTTCAAACATG6544–6566
5′7550/5′-GGTCCCCGTCAACCCAGAGAAT7522–7543
/3′ORF1b5′-ACCGTCGACATTCATCATACCTA7568–7590
/3′82425′-CGGATAATGGGACAGGTTC7633–7652
/3′42-605′-AATCCTTTCACCCGCATCA8123–8141
5′40-56/5′-CCTGATGCGGGTGAAAG8139–8156
5′8342/5′-AGGAGMAYTGGCAAACYGTGAC8352–8375
5′8242/5′-CCCGCYAAGACCTCKATGG8539–8560
VRBP1/5′-GTTTGGTGATCTATGCACAG9080–9099
5′-160/5′-TCCCACCATGCCAAACTATCACTG9237–9260
/VRBP25′-CAGATGTTCAACCCACCAGT9257–9277
VRBP5/5′-CTCATGGACAGCTGTGCTTG9463–9482
/3′9655′-GTGGGCCGATGATGAACCTG10028–10047
VRBP9/5′-GAATGCACGGTTGCTCAGGC10840–10859
5′1500/5′-GAGGACGACGCCATCACTAT10588–10787
/VRBP65′-CCCAGGAGTGCCTAGAAAC11163–11181
/3′22605′-GGTGACATCCTCCAACGGTAGTGC11325–11348
/VRBP45′-GTTCAATGACAGGGCCCGG12031–12049
/P235′-GCCACCACATCCAAACTAC12489–12507