Table 1.

Nuclear export of 9-kb Rev-dependent HIV-1 mRNA

Cell linePlasmid transfectedCytoplasmic mRNA (% of total)a
HeLapNL4-326
U251MGpNL4-324
HeLapNL4-3Rev−0.8
U251MGpNL4-3Rev−0.5
  • a The percent accumulation of 9-kb HIV-1 mRNA detected in the cytoplasmic fraction relative to that in the total-cell RNA of astrocytes and HeLa cells transfected with pNL4-3 and pNL4-3Rev− (Fig. 2B and C) was determined from densitometric analysis of Northern blot pNL4-3Rev− assays.