Table 1.

Number of mice showing herpetic lesions after HSV-2 infection

Immunization groupNo. of mice with herpetic lesions/no. survivinga (%) at the following day after HSV-2 challenge:
16202428354260
pgD + pCDNA5/5 (100)5/5 (100)4/5 (80)4/5 (80)3/5 (60)2/5 (40)2/5 (40)
pgD + IL-83/7 (43)2/7 (29)2/7 (29)1/7 (14)1/7 (14)0/7 (0)0/7 (0)
pgD + RANTES5/7 (71)4/7 (57)3/7 (43)3/7 (43)2/7 (29)1/7 (14)0/7 (0)
  • a Each group of mice (n = 8) was immunized with gD DNA vaccines (60 μg) plus chemokine cDNAs (40 μg) at 0 and 2 weeks. Three weeks after the last DNA injection, mice were challenged i.vag. with 200 LD50 of HSV-2 strain 186 (7 × 105 PFU). Surviving mice were checked every day after viral challenges to observe the pathological symptoms.