Table 2.

Ras-GTP levels in virus infected cellsa

InfectionMean Ras-GTP level ± SEM at time (h) p.i.:
024812
HSV-23.0 ± 0.112.1 ± 0.219.1 ± 0.415.0 ± 0.63.3 ± 0.5
ICP10ΔPK4.1 ± 0.53.5 ± 0.43.5 ± 0.33.1 ± 0.53.9 ± 0.2
HSV-2(R)2.8 ± 0.2ND18.4 ± 0.613.2 ± 0.43.0 ± 0.1
Mock (10% FCS)3.9 ± 0.73.4 ± 0.43.9 ± 0.33.5 ± 0.63.2 ± 0.4
Mock (1% FCS)3.6 ± 0.63.1 ± 0.34.0 ± 0.13.1 ± 0.23.5 ± 0.3
  • a Vero cells were labeled with [32P]orthophosphate for 18 h in medium containing 1 or 10% FCS and infected with HSV-2 or HSV-2(R) (in 1% FCS), or ICP10ΔPK (in 10% FCS) (73) or mock infected with PBS (in 1 or 10% FCS). Extracts obtained at various times p.i. were precipitated with Ras antibody and the levels of Ras.GTP and Ras.GDP in the precipitates were determined after separation on PEI cellulose thin-layer plates.