Table 3.

Inhibition of HCV E2-CD81 binding by HCV HMAbs

AntibodyNOB 1aaMean OD CD81-E2/GST-E2b
Q1aQ1bQ2aQ2b
CBH-251.51.11.71.2
CBH-521.41.12.11.2
CBH-772.61.22.61.2
CBH-8C101.51.12.11.4
CBH-8E81.41.11.51.3
CBH-1131.21.12.01.3
CBH-4G18.316.510.815.1
CBH-4B25.217.14.67.6
CBH-4D23.311.52.04.0
CBH-178.610.21.11.1
R041.11.01.21.1
  • a HMAb reactivity in representative NOB assays, presented as the concentration (micrograms per milliliter) of antibody that results in 50% inhibition of binding of 1 μg of HCV 1a E2 to 105 CD81-expressing Molt-4 cells. Antibodies were tested at concentrations that ranged from 0.1 to 300 μg/ml. —, negative.

  • b Aliquots of BSC-1 cell extracts containing approximately 400 ng of HCV E2 protein were combined with HCV HMAbs (5 μg/ml) in 100 μl of BLOTTO and added to wells coated with 100 ng of GST–CD81-LEL fusion protein or GST as described in Materials and Methods. Values are the mean OD value of antibody captured by CD81 divided by the mean OD value for antibody captured by GST in the presence of the indicated E2 protein. OD values obtained from wells coated with GST ranged between 0.021 and 0.081.