Table 2.

Comparison of CTL assays

VolunteerResult of:
IFN-γ ELISPOT assay (total no. of SFC/106 PBMCs [background])SL9 tetramer assay (% of CD8+ T cells responding)Gag lytic assay (% of bulk lysis specific for rVV Gaga)
rVV GagGag poolSL9 peptide
C125 (21)0 (0)0 (0)NDb 4
C29 (5)0 (0)0 (0)ND0
C32 (2)0 (0)14 (0)ND1
C44 (4)1 (0)0 (0)ND3
C50 (0)0 (0)1 (0)ND4
C62 (0)6 (4)4 (4)NDND
C70 (0)0 (7)NDND1
P116 (5)12 (4)100 (4)0.1112
P2123 (66)6 (3)36 (2)0.4525
P3186 (8)64 (1)72 (1)0.1445
P432 (10)295 (10)139 (10)0.2426
P5193 (80)20 (2)61 (2)0.2668
P6240 (62)337 (1)12 (1)0.01ND
P724 (7)314 (0)64 (0)0.1360
  • a Shown at the 50:1 E:T ratio.

  • b ND, not done.