Table 1.

Primers used for mutagenesis

OrientationSequenceaAmino acid mutation(s)
Sense5′-TCTTACGTACACAATGTGTAAGAAAACAAAGTT-3′308Asp→Lys
Sense5′-TCTTACGTACACAATGTGTGACTTAACAAAGTT-3′309Lys→Leu
Sense5′-TCTTACGTACACAATGTGTGACA-Δ-CAAAGTTCACATGG-3′Δ309
Sense5′-TCTTACGTACACAATGTGTGACAAAAAAAAGTTCAC-3′310Thr→Lys
Sense5′-TCTTACGTACACAATGTGTGACAAAACAGAGTTCACAT-3′311Lys→Glu
Sense5′-TCTTACGTACACAATGTGTAAGAAAACAGAGTTCACAT-3′308Asp→Lys and 311Lys→Glu
Sense5′-TCTTACGTACACAATGTGTGACAAAAAAGAGTTCACAT-3′310Thr→Lys and 311Lys→Glu
Antisense5′-CTGACTGGGATCCTACAGGG-3′
  • a Restriction enzyme recognition sequences are shown in italics; nucleotide substitutions are underlined; “Δ” indicates a three-nucleotide deletion.