Table 1.

Primer pairs and restriction enzymes used for RFLP assays

RegionGenomic primer: nucleotide positionaComplementary primer: nucleotide positionaLength (bp)RFLP assayRestriction enzymes
5′UTRUG52: (162)-CAAGCACTTCTGTTTCCCCGG-(182)UC53: (595)-TTGTCACCATAAGCAGCCA-(577)4345′ HaeIII,AluI, HpaII
VP4UG53: (577)-TGGCTGCTTATGGTGACAAT-(595)UC21: (1200)-TCAGGTAATTTCCACCACCA-(1181)6244 AluI,HpaII, DdeI
VP2UG21: (1172)-TCGAGAGGGTGGTGGTGGAA-(1191)UC24: (1923)-ATCATGGTGTCTATTTCTGC-(1904)7522 AluI,DdeI, AluI
VP3UG24: (1913)-GACACCATGATTCCCCTTAA-(1932)UC20: (2421)-TCATTACACGCTGACACAAA-(2402)5093 HaeIII,RsaI, TaqI
VP1UG1: (2402)-TTTGTGTCAGCGTGTAATGA-(2421)UC1: (2881)-GAATTCCATGTCAAATCTAGA-(2862)4801 HaeIII,DdeI, HpaII
VP1-2AUG19: (2870)-GACATGGAATTCACCTTTGTGG-(2891)UC13: (3648)-TAGTACTTAGCTTCCATGTA-(3629)7791-2A HinfI,RsaI, Bsp1286
P2UG13: (3617)-CCCACCTTCCAGTACATGGA-(3636)UC14: (4842)-CTGGCCAGTGCATCACTGTG-(4823)1,226P2 HaeIII,HinfI, DdeI
2CUG23: (4169)-AAGGGATTGGAGTGGGTGTC-(4188)UC15: (4965)-CATCTCTTGAAGTTTGCTGG-(4946)7972C AluI,HinfI, RsaI
3AB-CUG15: (4936)-CTGTCACCAACCAGCAAACTT-(4956)UC16: (5940)-TGTGAACCGTTCCCACCAAC-(5921)1,0053A-C HaeIII,HinfI, RsaI
3DUG7: (6086)-TTTGAAGGGGTGAAGGAACCAGC-(6108)UC8: (6376)-GATGTCTCTCTTCTTCTTTCCC-(6355)2913D1 HaeIII,DdeI, RsaI
3C-DUG16: (5921)-GTTGGTGGGAACGGTTCACA-(5940)UC12: (6516)-TCAATTAGTCTGGATTTTCCCTG-(6494)5963D3 HinfI,RsaI, Dde
3D-3′UTRUG17: (6536)-TCAGTGGCCATGAGAATGGC-(6555)UC10: (7464)-TTTTTTTTTTTTTTTTTTTTTTTC-(7441)9293D-3′ HinfI,DdeI, HpaII
  • a Numbers refer to the nucleotide position of the primer on the Sabin 1 PV strain.