Table 1.

Bacterial strains, plasmids, phages, and primers

MaterialDescriptionReference or source
Bacterial strains
S. pneumoniae
  949Lysogenic for phage MM1; serotype 23F 40
  496Serotype 23F 40
  499Serotype 23F 40
  622Serotype 23F 40
  8249Serotype 19A 32
  CSUB 3409Serotype 9VJ. Liñares
  CSUB 4086Serotype 14J. Liñares
  SSISP33C/1Serotype 33CStatens Seruminstitut
  SSISP33F/1Serotype 33FStatens Seruminstitut
  746Lysogenic for phage HB-746; serotype 8 47
  708Hex 53
  M24S3 lytAHex 20
  M222Hex 17
  PM11708 pIAPU1 integrant strainThis study
E. coli DH5α supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 50
Plasmids
 pUCE191pUC derivative; Lnr; 4.1 kb 1
 pCE3pBR325 derivative; Cmr; harbors the 5′ moiety oflytA 16
 pIAPU1pUCE191::EcoRI-PstI MM1int-attP; 5.6 kbThis study
Phages
 MM1Temperate phage from strain 949This study
 HB-746Temperate phage from strain 746 47
 Dp-1Virulent phage 34
 Cp-1Virulent phage 48
 EJ-1Temperate phage from strain 101/87 13
 ω2Virulent phage 55
Primers
 EGP25′-GCAATTATATTCATTTTCTCTCC-3′This study
 EGP45′-GAAGATAGGAGGATAAACTGG-3′This study
 EGP85′-GGAATTCCCCACACTCAAATTTTGGC-3′This study
 EGP95′-AACTGCAGAAATTGTTCTTTCACCGCAGG-3′This study
 EGP145′-CCATCAAGACACCATTCGCC-3′This study
 EGP155′-CATATTGTAGACCATCGAGGC-3′This study