Table 1.

Antibody-independent infection of cells with DV2

Cell lineCell type% Positive cellsaMOI50b
Epithelial lineage
 BHK21-15Hamster kidney72.6
 SW13Adrenal adenocarcinoma73.6
 293Embryonal kidney90.50.05
 HeLaEpithelioid carcinoma62.11.0
Myeloid lineage
 U937Histiocytic lymphoma<0.2
 U937 + DMSO (1.25%)3.7
 U937 + PMA (16 nM)2.1
 HL-60Promyelocytic leukemia0.3
 HL-60 + DMSO (1.25%)2.4
 HL-60 + PMA (16 nM)1.0
 Monomac-6Myelomonocytic leukemia<0.2
 Monocyte-derived macrophages<0.2
Hematopoietic lineage
 JYEBVc B-cell lymphoma1.3
 SKW3T-cell leukemia<0.2
Fibroblast lineage
 L929Murine fibroblast78.00.5
 COS-7Monkey kidney fibroblast81.00.3
Endothelial cell lineage
 HUVECHuman umbilical vein endothelial cell12.05.0
  • a Human cells (unless specified) were exposed to DV2 (strain 16681), cultured for 72 h, harvested, and subjected to flow cytometry with MAbs against the E protein. The percentage of positive cells reflects expression of DV2 antigens after infection at the maximal input virus concentration (MOI, 10). Data are expressed as the average percentages of cells infected and are from at least two experiments per cell type. The detection of 0.2% positive cells approaches the sensitivity of this assay.

  • b For cells with appreciable levels of infection, a dose-response curve was conducted over a range of virus concentrations (MOI, 0.1 to 10.0). MOI50, relative virus concentration that caused infection at 50% of the maximum rate.

  • c EBV, Epstein-Barr virus.