Table 2.

Lymph node T lymphocytes from SIV gag plasmid DNA-vaccinated monkeys demonstrate Gag epitope-specific CTL responses 10 weeks following the initial immunization

ImmunizationMonkey% CD8αβ tetramer binding% Specific lysisc
FreshaPeptide stimulatedb
SIVgag P0910.117.265.7
N5290.01.013.0
T2580.012.735.7
92980.128.136.5
257NDd NDND
ControlP9670.01.34.1
R4680.00.26.5
T7200.00.23.2
V2990.03.32.1
  • a Lymphocytes from freshly obtained lymph nodes were directly assessed for binding of the Mamu-A*01/p11C tetramer to CD8αβ+ T cells.

  • b Lymph node cells were cultured in vitro with p11C (10 μg/ml) for 12 days and then assessed for binding of the Mamu-A*01/p11C tetramer to CD8αβ+ T cells.

  • c Lymph node cells were cultured in vitro with p11C (10 μg/ml) for 12 days and then assessed for p11C-specific lysis using a standard 51Cr release assay. Data represent percent specific lysis at an effector-to-target ratio of 5:1.

  • d ND, not done.