Table 1.

SIV Gag epitope p11C-specific CD8+T-lymphocyte responses after plasmid DNA immunization

ImmunizationMonkeyAssayValue at indicated wk after initial immunization
04689101216242526282930313234
SIVgag P091% Fresha 0.00.00.30.10.10.20.20.50.30.90.60.1NDf NDNDNDND
No. of cells/μl by tetramerb NDND4ND1NDNDND365NDNDNDNDNDND
No. of cells/μl by LDAc 00.040.160.460.020.37NDNDNDNDNDNDNDNDNDNDND
% Peptide stimulatedd 0.111.340.623.340.847.464.875.127.320.951.454.0NDNDNDNDND
% Specific lysise 0.046.546.348.451.047.173.682.264.046.458.362.5NDNDNDNDND
N529% Fresh0.00.00.00.00.00.00.00.00.00.00.10.0NDNDNDNDND
No. of cells/μl by tetramerNDNDNDND0NDNDND0010NDNDNDNDND
No. of cells/μl by LDA0.00.00.00.00.00.0NDNDNDNDNDNDNDNDNDNDND
% Peptide stimulated0.10.30.40.11.30.40.5ND0.20.20.40.7NDNDNDNDND
% Specific lysis0.06.86.41.619.43.66.6ND6.111.613.018.2NDNDNDNDND
T258% Fresh0.00.10.10.00.10.10.10.00.10.10.10.20.30.10.10.10.0
No. of cells/μl by tetramerNDND1ND1NDNDND0224521ND0
No. of cells/μl by LDA0.00.00.00.00.00.0NDNDNDNDNDNDNDNDNDNDND
% Peptide stimulated0.111.57.97.513.321.121.515.18.66.813.117.420.019.714.23.43.6
% Specific lysis0.022.432.828.250.338.743.556.651.639.247.169.268.380.665.2ND55.9
9298% Fresh0.00.00.10.00.00.20.10.10.10.10.30.00.20.10.20.20.1
No. of cells/μl by tetramerNDND1ND0NDNDND113031221
No. of cells/μl by LDA0.00.00.00.00.00.0NDNDNDNDNDNDNDNDNDNDND
% Peptide stimulated1.125.032.518.426.934.126.219.319.721.732.025.930.126.112.814.816.7
% Specific lysis7.526.547.727.843.528.248.131.461.037.841.352.155.150.547.5ND55.9
257% Fresh0.00.20.7ND0.10.30.1ND0.00.11.1NDNDNDNDNDND
No. of cells/μl by tetramerNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
No. of cells/μl by LDANDNDNDNDNDNDNDNDNDNDNDNDNDNDNDNDND
% Peptide stimulatedND36.0NDNDND30.832.4ND29.313.815.3NDNDNDNDNDND
% Specific lysis0.055.0NDNDND47.173.6ND52.053.945.3NDNDNDNDNDND
ControlP967% FreshND0.0ND0.00.00.00.00.00.00.00.00.0NDNDNDNDND
No. of cells/μl by tetramerNDNDNDND0NDNDND000NDNDNDNDNDND
No. of cells/μl by LDA0.00.00.00.00.00.0NDNDNDNDNDNDNDNDNDNDND
% Peptide stimulated0.30.00.06.60.30.20.23.00.81.80.20.2NDNDNDNDND
% Specific lysis0.00.00.00.03.50.00.00.00.00.09.10.0NDNDNDNDND
R468% FreshND0.0ND0.00.00.00.00.00.00.00.00.0NDNDNDNDND
No. of cells/μl by tetramerNDNDNDND0NDNDND000NDNDNDNDNDND
No. of cells/μl by LDA0.00.00.00.00.00.0NDNDNDNDNDNDNDNDNDNDND
% Peptide stimulated0.20.00.00.00.30.20.22.70.70.50.40.2NDNDNDNDND
% Specific lysis0.00.00.00.04.60.00.70.00.06.43.00.0NDNDNDNDND
T720% Fresh0.00.00.00.00.00.00.00.00.00.00.00.00.00.00.00.00.0
No. of cells/μl by tetramerNDND0ND0NDNDND000000000
No. of cells/μl by LDA0.00.00.00.00.00.0NDNDNDNDNDNDNDNDNDNDND
% Peptide stimulated0.30.10.17.64.12.31.01.31.01.11.50.10.11.00.90.30.4
% Specific lysis11.00.00.00.04.50.01.70.011.819.50.08.01.27.66.1ND1.4
V299% Fresh0.00.00.00.00.00.00.00.00.00.00.00.0NDNDNDNDND
No. of cells/μl by tetramerNDND0ND0NDNDND0000NDNDNDNDND
No. of cells/μl by LDA0.00.00.00.00.00.0NDNDNDNDNDNDNDNDNDNDND
% Peptide stimulated0.00.15.71.10.20.10.71.00.62.02.01.7NDNDNDNDND
% Specific lysis3.90.05.40.05.70.00.00.813.66.95.1NDNDNDNDNDND
  • a CD8αβ+ PBL in whole blood were assessed for binding of the Mamu-A*01/p11C tetramer.

  • b Total number of p11C-specific CD8αβ+ cells per microliter of whole blood as calculated by binding of the Mamu-A*01/p11C tetramer.

  • c Total number of p11C-specific CD8αβ+ cells per microliter of whole blood as calculated by LDA.

  • d PBL were cultured in vitro with p11C (10 μg/ml) for 12 days and then assessed for binding of the Mamu-A*01/p11C tetramer to CD8αβ+ T cells.

  • e PBL were cultured in vitro with p11C (10 μg/ml) for 12 days and then assessed for p11C-specific lysis using a standard 51Cr release assay. Data represent percent p11C-specific lysis at an effector-to-target ratio of 20:1.

  • f ND, not done.