Table 1.

Transduction of dividing CaCo-2 and DGBE cellsa

Cells and vector% GFP-positive cellsMean fluorescence intensity
CaCo-2
 Untransfected2.523.1
 pLNCeGFP44140.6
 pRtatpeGFP992,781
 pRRLcmveGFPsin76287.6
DGBE
 Untransfected122.6
 pLNCeGFP892,229
 pRtatpeGFP872,498
 pRRLcmveGFPsin961,609
  • a Subconfluent CaCo-2 and DGBE cells were infected with murine retrovirus LNCeGFP, first-generation lentivirus pRtatpeGFP, and third-generation lentivirus pRRLcmveGFPsin. Cells were analyzed by flow cytometry. All viral vectors transduced target cells efficiently. Mean fluorescence levels are similar in DGBE cells, and pRtatpeGFP mediates higher expression levels in CaCo-2 cells.