Table 3.

SIV Gag epitope p11C-specific CD8+T-lymphocyte responses of vaccinated animals after intravenous challenge with SIVsm E660

ImmunizationMonkeyAssayValue at indicated day after challenge
037101214172124283542496377
SIVgag P091% Fresha 0.10.10.010.557.356.937.725.3NDe 14.410.45.73.26.85.5
No. of cells/μlb 1103062,843822856760ND24115665396846
% Peptide stimulatedc 54.048.162.220.734.476.059.584.5ND87.070.375.883.383.390.8
% Specific lysisd 62.562.380.6NDND73.579.887.1ND78.7ND98.181.575.859.0
N529% Fresh0.00.00.01.09.111.24.53.7ND1.43.03.23.12.21.8
No. of cells/μl000878915258ND9382136ND21
% Peptide stimulated0.70.30.99.412.65.56.215.3ND5.12.75.112.228.725.9
% Specific lysis18.27.46.0ND33.318.745.468.5ND34.3ND60.252.762.153.6
T258% Fresh0.10.00.10.10.10.10.22.03.83.02.11.10.90.60.8
No. of cells/μl1021123257661352011611
% Peptide stimulated3.62.411.96.74.211.77.013.215.825.68.425.718.820.513.9
% Specific lysis23.5ND60.839.721.253.350.554.471.055.950.141.445.442.338.1
9298% Fresh0.10.29.117.113.09.08.55.45.64.63.82.52.42.11.7
No. of cells/μl1162210176146856660501322201512
% Peptide stimulated16.73.222.527.118.440.446.013.827.937.60.040.126.013.626.6
% Specific lysis55.9ND56.065.541.871.077.851.377.752.658.045.837.113.142.3
ControlP967% Fresh0.00.00.00.00.41.80.90.7ND0.60.91.91.43.31.7
No. of cells/μl000022798ND5810103117
% Peptide stimulated0.20.20.30.245.737.560.263.9ND51.54.517.914.935.746.8
% Specific lysis0.03.40.00.046.030.161.667.2ND48.4NDND26.539.640.4
R468% Fresh0.00.00.00.00.63.31.30.6ND0.60.40.20.20.4f
No. of cells/μl0000111032311ND84225
% Peptide stimulated0.20.10.20.410.136.145.946.6ND18.93.715.76.314.3
% Specific lysis0.02.52.50.036.449.970.180.4ND40.2ND62.033.556.0
T720% Fresh0.00.00.00.00.00.11.61.81.30.80.50.50.70.90.8
No. of cells/μl00000138323418106693
% Peptide stimulated0.40.80.20.30.12.926.917.928.821.01.616.78.04.26.3
% Specific lysis1.4ND4.70.02.114.667.154.477.048.415.039.023.51.57.7
V299% Fresh0.00.00.00.10.00.31.21.0ND0.70.71.01.71.41.7
No. of cells/μl00000298ND245141013
% Peptide stimulated1.70.34.80.52.652.641.638.9ND5.631.821.329.417.420.5
% Specific lysis13.8ND7.20.74.272.874.662.0NDND47.652.061.054.355.4
J8N% Fresh0.00.00.00.00.00.24.31.12.52.22.93.96.15.6ND
No. of cells/μl0ND00001939910192725ND
% Peptide stimulated0.60.50.30.50.40.74.09.34.72.5ND13.83.72.80.0
% Specific lysis3.7ND4.70.55.6NDND24.6ND3.46.719.70.73.52.8
  • a CD8αβ+PBL in whole blood were assessed for binding of the Mamu-A*01/p11C tetramer.

  • b Total number of CD8αβ+ cells binding the Mamu-A*01/p11C tetramer per microliter of whole blood.

  • c PBL were cultured in vitro with p11C (10 μg/ml) for 12 days and then assessed for binding of the Mamu-A*01/p11C tetramer to CD8αβ+ T cells.

  • d PBL were cultured in vitro with p11C (10 μg/ml) for 12 days and then assessed for p11C-specific lysis using a standard 51Cr release assay. Data represent percent p11C-specific lysis at an effector-to-target ratio of 10:1.

  • e ND, not done.

  • f Animal was euthanized.