Table 1.

Mutagenic oligodeoxynucleotides used in this study

MutantMutagenic oligodeoxynucleotide
DesignationSequence
P2-PYP2-PY-F5′-ACAGCCTCGGCTCCTCCTCCTCCTTATGTGGGGAGGGACCCAAGACCACC-3′
P2-PY-R5′-CACATAAGGAGGAGGAGGAGCCGAGGCTGTGTCTTCTGTAAGTAGGTC-3′
P6-PYP6-PY-F5′-GAGCCAACAGCCCCACCAGAAGAGAGCTTCAGGTCTGGGAGGGACCCAAGACCACC-3′
P6-PY-R5′-TTCTGGTGGGGCTGTTGGCTCTGGTCTGCTCTGAAGGTCTTCTGTAAGTAGGTC-3′
PY-P12PY-P12-F5′-CTACTTACAGAAGACCCCCCGCCTTATAGGGACGGAGAGGCACCGGACCC-3′
PY-P12-R5′-CCTATAAGGCGGGGGGTCTTCTGTAAGTAGCGCAGGGGTCGCTTCTC-3′
P2-P12P2-P12-F5′-ACAGCCTCGGCTCCTCCTCCTCCTTATGTGGGGGGAGAGGCACCGGACCC-3′
P2-P12-R5′-CACATAAGGAGGAGGAGGAGCCGAGGCTGTCGCAGGGGTCGCTTCTC-3′
P6-P12P6-P12-F5′-GAGCCAACAGCCCCACCAGAAGAGAGCTTCAGGTCTGGGGGAGAGGCACCGGACCC-3′
P6-P12-R5′-TTCTGGTGGGGCTGTTGGCTCTGGTCTGCTCTGAAGCGCAGGGGTCGCTTCTC-3′
PY-MAPY-MA-F5′-CTACTTACAGAAGACCCCCCGCCTTATAGGGACACCCCGCCTCGATCCTC-3′
PY-MA-R5′-CCTATAAGGCGGGGGGTCTTCTGTAAGTAGCGAACGAGGAGGTTCAAG-3′
P2-MAP2-MA-F5′-ACAGCCTCGGCTCCTCCTCCTCCTTATGTGGGGACCCCGCCTCGATCCTC-3′
P2-MA-R5′-CACATAAGGAGGAGGAGGAGCCGAGGCTGTCGAACGAGGAGGTTCAAG-3′
P6-MAP6-MA-F5′-GAGCCAACAGCCCCACCAGAAGAGAGCTTCAGGTCTGGGACCCCGCCTCGATCCTC-3′
P6-MA-R5′-TTCTGGTGGGGCTGTTGGCTCTGGTCTGCTCTGAAGCGAACGAGGAGGTTCAAG-3′
PY-NCPY-NC-F5′-CTACTTACAGAAGACCCCCCGCCTTATAGGGACACCTCCCTCCTGACCCTAG-3′
PY-NC-R5′-CCTATAAGGCGGGGGGTCTTCTGTAAGTAGCTGGGGTCTTGGTCCCC-3′
P2-NCP2-NC-F5′-ACAGCCTCGGCTCCTCCTCCTCCTTATGTGGGACCTCCCTCCTGACCCTAG-3′
P2-NC-R5′-CACATAAGGAGGAGGAGGAGCCGAGGCTGTCTGGGGTCTTGGTCCCC-3′
P6-NCP6-NC-F5′-GAGCCAACAGCCCCACCAGAAGAGAGCTTCAGGTCTGGGACCTCCCTCCTGACCCTAG-3′
P6-NC-R5′-TTCTGGTGGGGCTGTTGGCTCTGGTCTGCTCTGAAGCTGGGGTCTTGGTCCCC-3′