Table 1.

PCR amplimers used to prepare truncated and mutated gI constructs

Construct 3′ primer
gIwt 5′-CCCTCTAGATAATTAGTTCTATTTAAC-3′
gIδ1 5′-CCCTCTAGATACAACGGAATGTGGGGG-3′
gIδ2 5′-CCCTCTAGATGGGGGGGATTCTTCGCG-3′
gIδ3 5′-CCCTCTAGATTCTTCGCGAATCGTTGC-3′
gIδ4 5′-CCCTCTAGATATTACAATAACAATAAC-3′
gIδ5 5′-CCCTCTAGATTGTGCAATGGCGGCCTC-3′
gIδ6 5′-CCCTCTAGATATGCCCCTTCTTGTTTT-3′
gIδ7 5′-CCCTCTAGATATTACAATAACAATAAC-3′
gIδ2KK 5′-CCCTCTAGAGGGGGATTTTTTGCGAATCGT-3′
gIδ3T→A 5′-CCCTCTAGAGCGAATCGCTGCTAGTTGTGC-3′
gIδ3T→S 5′-CCCTCTAGAGCGAATCGATGCTAGTTGTGC-3′
gIδ3T→D 5′-CCCTCTAGAGCGAATGTCTGCTAGTTGTGC-3′
gIδ3R→E 5′-CCCTCTAGACTCAATCGTTGCTAGTTGTGC-3′