Table 2.

Absence of transcytosis of HAV in Caco-2 cells

Inoculum (2.1 × 106 RFU)Elapsed time (h)aAmt of contralateral supernatant virus (RFU)% Transcytosisb
Apical2360.0017
<36<0.0017
<36<0.0017
24720.0034
<36<0.0017
Basolateral2<8<0.0004
<8<0.0004
<8<0.0004
  • a Replicate polarized cultures of Caco-2 cells were inoculated with virus on the indicated surface; after the time indicated, the virus content of the contralateral supernatant fluids was measured.

  • b Transcytosis is reported as the percentage of the original inoculum present within the contralateral medium at 2 or 24 h.