Table 1.

Quantitative analysis of Vero cells infected with various strains of HSV-1

ParameterStrain
KOS8MA/ΔSma8MA
No. of cells counted203030
No. of cells with visible viral particles191912
No. of capsids/cell counted in:
 Nucleus55.68.32.2
 Cytoplasm6.10.90.2
No. of enveloped particles/cell counted in:
 Perinuclear space6.52.70.2
 Cytoplasm1.100
 Extracellular space2300
Total no. of viral particles/cell counted92.311.92.6
Total no. of viral particles relative to KOS (%)100133
% Packaged DNAa 100314
  • a Amount of packaged DNA relative to KOS as calculated from the total signal of samples treated with DNase (see Fig. 2, experiment 1).